Biofungicide Formulation Based on Bacillus velezensis EM-A8 for Control of Maize Foliar Diseases
DOI:
https://doi.org/10.48162/rev.39.196Keywords:
biological control, formulation, growth medium, Northern leaf blightAbstract
The aim was to evaluate inoculum production of Bacillus velezensis EM-A8, a native bacterium of maize phyllosphere, antagonist to foliar pathogens Exserohilum turcicum and Puccinia sorghi. Six economic media were tested: 1) Nutrient Broth (8 g.L-1); 2) Whole soybean flour (40 g.L-1) + sucrose (20 g.L-1); 3) Whole soybean flour (10 g.L-1) + molasses (20 g.L-1), 4) Whole soybean flour (40 g.L-1) + molasses (10 g.L-1); 5) Yeast extract (10 g.L-1) + molasses (5 g.L-1) and 6) Yeast extract (10 g.L-1) + sucrose (5 g.L-1). Growth was determined spectrophotometrically at 620 nm and viability was estimated. M2 showed the shortest generation time (g 1.22 h). M3 and M5 were selected for efficiency and aW was modified with glycerol at 0.97. Inoculums were stored under refrigeration (5°C) and room temperature (20-25°C) for 8 months. At 5°C the viability of the antagonist at 3.3-4.5 log CFU ml-1 was significantly lower than at room temperature (6-6.8 log CFU ml-1), where M5 showed the highest stability. The bioformulation of B. velezensis EM-A8 in M5 at aW 0.97 and stored at room temperature will allow successful control of maize foliar diseases.
Highlights:
- Commercial products and by-products in different media provide high growth and shelf life of velezensis EM-A8
- Storage temperature significantly affected velezensis EM-A8 viability
- velezensis EM-A8 in growth medium with yeast extract and molasses, modified aW with glycerol at 0.97 and stored at room temperature is a promising product
- Liquid formulations stored at room temperature maintained stable viability in 6 log for 8 months.
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References
Babbal, A.; Khasa, Y. 2017. Microbes as biocontrol agents. In: Probiotics and Plant Health. Kumas, V. (Eds). Springer. 507-551.
Barkai-Golan, R. 2001. Postharvest diseases of fruits and vegetables. Development and control. Elsevier, Amsterdam, The Netherlands.
Bonaterra, A.; Badosa, E.; Daranas, N.; Francés, J.; Roselló, G.; Montesinos, E. 2022. Bacteria as biological control agents of plant diseases. Microorganisms. 10: 1759.
Boza, Y.; Barbin, D.; Scamparini, A. 2004. Effect of spray drying on the quality of encapsulated cells of Beijerinckia sp. Process Biochemistry. 39: 1275-1284.
Costa, E.; Teixidó, N.; Usall, J.; Atarés, E.; Viñas, I. 2001. Production of the biocontrol agent Pantoea agglomerans strain CPA-2 using commercial products and byproducts. Applied Microbiology and Biotechnology. 56: 367-371.
Costa, E.; Teixidó, N.; Usall, J.; Atarés, E.; Viñas, I. 2002. The effect of nitrogen and carbon sources on growth of the biocontrol agent Pantoea agglomerans strain CPA-2. Letters in Applied Microbiology. 35: 117-120.
Dallyl, H.; Fox, A. 1980. Spoilage of material of reduced water activity by xerophilic fungi. Technical Series 15. London: Academic Press. 219-239.
Di Rienzo, J.; Casanoves, F.; Balzarini, M.; Gonzalez, L.; Tablada, M.; Robledo, C. 2012. InfoStat versión. Argentina: GrupoInfoStat, FCA, Universidad Nacional de Córdoba. http://www.infostat. com.ar
Fessia, A.; Sartori, M.; García, D.; Fernández, L.; Ponzio, R.; Barros, G.; Nesci, A. 2022. In vitro studies of biofilm-forming Bacillus strains, biocontrol agents isolated from the maize phyllosphere. Biofilm 4, 100097.
Fonseca, F.; Cenard, S.; Passot, S. 2015. Freeze-drying of lactic acid bacteria. In: Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology, vol 1257. Wolkers, W.; Oldenhof, H. (eds) Springer, New York,
Kinay, P.; Yildiz, M. 2008. The shelf life and effectiveness of granular formulations of Metschnikowia pulcherrima and Pichia guilliermondii yeast isolates that control postharvest decay of citrus fruit. Biological Control. 45: 433-440.
Kumar, S.; Thakur, M.; Rani, A. 2014. Trichoderma: Mass production, formulation, quality control, delivery and its scope in commercialization in India for the management of plant diseases. African Journal of Agricultural Research. 9(53): 3838-3852.
Reis, F; Servulo, E.; De Franca, F. 2004. Lipopeptide surfactant production by Bacillus subtilis grown on low-cost raw materials. Applied Biochemistry and Biotechnology. 113: 899-912.
Sartori, M.; Nesci, A.; Etcheverry, M. 2012. Production of Fusarium verticillioides biocontrol agents, Bacillus amyloliquefaciens and Microbacterium oleovorans, using different growth media: evaluation of biomass and viability after freeze-drying. Food Additives and Contaminants (A). 29(2): 287-292.
Sartori, M.; Nesci, A.; Formento, A.; Etcheverry, M. 2015. Selection of potential biological control of Exserohilum turcicum with epiphytic microorganisms from maize. Revista Argentina de Microbiología. 47(1): 62-71.
Sartori, M.; Nesci, A.; Garcia, J.; Passone, M.; Montemarani, A.; Etcheverry M. 2017a. Efficacy of epiphytic bacteria to prevent northern leaf blight caused by Exserohilum turcicum in maize. Revista Argentina de Microbiología. 49(1): 75-82.
Sartori, M.; Nesci, A.; Montemarani, A.; Barros, G.; Garcia, J.; Etcheverry M. 2017b. Preliminary evaluation of biocontrol agents against maize pathogens Exserohilum turcicum and Puccinia sorghi in field assays. Agricultural Sciences. 8: 1003-1013.
Sartori, M.; Bonacci, M.; Barra, P.; Fessia, A.; Etcheverry, M.; Nesci, A.; Barros, G. 2020. Studies on possible modes of action and tolerance to environmental stress conditions of different biocontrol agents of foliar diseases in maize. Agricultural Sciences. 11: 552-566.
Schisler, D.; Slininger, P.; Behle, R.; Jackson, M. 2004. Formulation of Bacillus spp. for biological control of plant diseases. Phytopathology. 94: 1267-1271.
Stanbury, P.; Whitaker, A.; Hall, S. 1995. Media for industrial fermentations. In: Principles of fermentation technology. Pergamon Press, Oxford. 93-121.
Tabbene, O.; Slimene, I.; Djebali, K.; Mangoni, M.; Urdaci M.; Limam F. 2009. Optimization of medium composition for the production of antimicrobial activity by Bacillus subtilis B38. Biotechnology Progress. 25: 1266-1274.
Teixidó, N.; Cañamás, T. P.; Abadias, M.; Usall, J; Solsona, C.; Casals, C.; Viñas, I. 2006. Improving low water activity and desiccation tolerance of the biocontrol agent Pantoea agglomerans CPA- 2 by osmotic treatments. Journal of Applied Microbiology. 101: 927-937.
Teixidó, N.; Usall, J.; Torres, R. 2022. Insight into a Successful development of biocontrol agents: production, formulation, packaging and shelf life as key aspects. Horticulturae. 8: 305.
Vandemeer, J. 1995. The ecological basis of alternative agriculture. Annual review of Ecology and Systematics. 26: 201-224.
Vermelho, A.; Moreira, J.; Junior, A.; da Silva, C.; Cardoso, V.; Akamine, I. 2024. Microbial preservation and contamination control in the baking industry. Fermentation. 10: 231. doi.org/10.3390/ fermentation10050231
Yánez-Mendizábal, V.; Viñas, I.; Usall, J.; Torres, R.; Solsona, C.; Teixidó, N. 2012. Production of the postharvest biocontrol agent Bacillus subtilis CPA-8 using low-cost commercial products and by-products. Biological control. 60: 280-289.
Younis, M.; Hezayen, F.; Nour-Eldein, M.; Shabeb, M. 2010. Optimization of cultivation medium and growth conditions for Bacillus subtilis KO strain isolated from sugar cane molasses. American Eurasian Journal of Agricultural Environmental Science. 7: 31-37.
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